Tanshinone IIA Inhibits Glutamate-Induced Oxidative Toxicity through Prevention of Mitochondrial Dysfunction and Suppression of MAPK Activation in SH-SY5Y Human Neuroblastoma Cells

Figure 5

Effect of tanshinone IIA on phosphorylation levels of JNK, ERK, and p38 MAPK in glutamate-exposed SH-SY5Y cells. (a) The cells were treated with tanshinone IIA at the indicated concentrations and then exposed to 10 mM glutamate. Western blot analysis was performed using the indicated antibodies with β-actin as a loading control. (b) Quantification of immunoblots was performed by densitometry analysis, and relative phosphorylation levels were expressed as the ratios of phospho-MAPK to total MAPK, respectively. The data are presented as mean ± SEM of three independent experiments. Tan IIA: tanshinone IIA; Glu: glutamate. compared to the cells exposed to glutamate alone.

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