SIRT1 Activation by Resveratrol Alleviates Cardiac Dysfunction via Mitochondrial Regulation in Diabetic Cardiomyopathy Mice

Figure 4

High glucose and SIRT1 deletion impaired mitochondrial biogenesis and function. (a and b) Both HG and SIRT1 deletion by shRNA lentiviral vector increased apoptotic cell ratio in H9c2 cells (), but resveratrol did not suppress HG-induced apoptosis in SIRT1^KD + HG + R cells as in HG + RES cells (^#, ^&). (c) Control cells showed strong TMRM fluorescence, while HG and SIRT1^KD resulted in decreased TMRM fluorescence due to depolarization of the mitochondria. Additionally, resveratrol relatively increased TMRM fluorescence in HG + RES cells but not in SIRT1^KD + HG + R cells. (d) mtDNA amount was reduced in HG and SIRT1^KD-treated cells (), and resveratrol markedly increased mtDNA amount in the HG + RES group but not in the SIRT1^KD + HG + R group (^#, ^&). (e) Mitochondrial enzyme activity was reduced in HG and SIRT1^KD-grouped cells (), and resveratrol markedly elevated it in the HG + RES group but not in the SIRT1^KD + HG + R group (^#, ^&). (f) ATP generation demonstrated the similar tendency with mitochondrial enzyme activity. versus Con; ^# versus DCM; ^& versus DCM + RES.

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