Reperfusion Therapy with Rapamycin Attenuates Myocardial Infarction through Activation of AKT and ERK

Figure 3

Effect of Rapamycin on myocardial apoptosis. (a) Representative images of TUNEL-positive nuclei in green fluorescent color and total nuclei staining with DAPI (blue) staining of sections of myocardium treated following 30 minutes of ischemia/24 hours of reperfusion. (b) Apoptotic index calculated as the % of TUNEL stained apoptotic nuclei versus the total amount of nuclei visualized via DAPI staining. (c) Representative immunoblots of Bax and Bcl-2 in hearts of C57 mice following 30 minutes of ischemia and 24 hours of reperfusion including Control (C57), DMSO (I/R + DMSO), and Rapamycin (I/R + RAPA) treated groups (/group). GAPDH immunoblot was used as a loading Control. (d) Densitometric analysis of the ratio of Bax to GAPDH (/group). (e) Densitometric analysis of the ratio of Bcl-2 to GAPDH (/group). (f) Densitometric analysis of the ratio of Bcl-2 to Bax (/group).

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