Reperfusion Therapy with Rapamycin Attenuates Myocardial Infarction through Activation of AKT and ERK

Figure 4

Rapamycin inhibits mTORC1 while simultaneously promoting mTORC2 activity. (a) Representative immunoblots of phosphorylated S6 (S235/236), total S6, phosphorylated AKT (S473), and total AKT in hearts of C57 mice following 30 minutes of ischemia and 1 hour and 24 hours of reperfusion including Control (C57), I/R + DMSO, and Rapamycin (I/R + RAPA) treated groups (/group). GAPDH immunoblots were used as a loading Control. (b) Densitometric analysis of the ratio of pS6 to S6. (c) Densitometric analysis of the ratio of S6 to GAPDH (/group). (d) Densitometric analysis of the ratio of pAKT to AKT (/group). (e) Densitometric analysis of the ratio of AKT to GAPDH (/group).

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