Reperfusion Therapy with Rapamycin Attenuates Myocardial Infarction through Activation of AKT and ERK

Figure 5

Effect of Rapamycin on MAP kinase signaling. (a) Representative immunoblots of phosphorylated ERK1/2, total ERK1/2, phosphorylated P38, and total P38 in hearts of C57 mice following 30 minutes of ischemia and 1 hour and 24 hours of reperfusion including Control (C57), DMSO (I/R + DMSO), and Rapamycin (I/R + RAPA) treated groups (/group). GAPDH immunoblots were used as a loading Control. (b) Densitometric analysis of the ratio of pERK1/2 to ERK1/2 (/group). (c) Densitometric analysis of the ratio of ERK1/2 to GAPDH (/group). (d) Densitometric analysis of the ratio of pP38 to P38. (e) Densitometric analysis of the ratio of P38 to GAPDH (/group).

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