Reperfusion Therapy with Rapamycin Attenuates Myocardial Infarction through Activation of AKT and ERK

Figure 7

Effect of ERK inhibitor on phosphorylation of AKT and ERK. (a) Representative immunoblots of phosphorylated AKT, total AKT, phosphorylated ERK1/2, total ERK1/2, and GAPDH in hearts of C57 mice following 30 minutes of ischemia and 24 hours of reperfusion including Control (C57), DMSO (I/R + DMSO), Rapamycin (I/R + RAPA), and PD (I/R + PD + RAPA) treated groups (/group). (b) Densitometric analysis of the ratio of pAKT/AKT, (c) AKT/GAPDH, (d) pERK1/2 to ERK1/2, and (e) ERK1/2 to GAPDH.

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