miR-382 Contributes to Renal Tubulointerstitial Fibrosis by Downregulating HSPD1

Figure 1

miR-382 contributed to the progression of renal tubulointerstitial fibrosis in UUO mice. (a) Staining of collagen, α-SMA, and Vimentin. (b) Relative abundance of miR-382. (c) The abundance of collagen was analyzed using Masson trichrome staining (Masson trichrome staining ×400, ). (d) The abundance of collagen was analyzed using Sirius red staining (Sirius red staining ×400, ). (e) Quantification of α-SMA staining. (f) Quantification of Vimentin staining. The abundance of collagen, α-SMA, or Vimentin was quantitatively measured using Image Pro-Plus 6.0 Software (Media Cybernetics, USA). Relative protein levels were expressed as ratios to control. (g) Serum creatinine concentration. Mice underwent unilateral ureteral obstruction or sham operation of the left ureter. Locked nucleic acid-modified anti-miR-382 oligonucleotides and antiscrambled were diluted in saline (5 mg/ml) and intravenously delivered via tail vein (10 mg/kg) within 30 min prior to UUO, and the dosage was repeated 24 hours after the surgery. ^Δ, compared with the normal group; ^∇, ^# compared with the UUO group. UUO indicates unilateral ureteral obstruction. Normal indicates the normal control group.

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