Metabolic Reprogramming, Autophagy, and Reactive Oxygen Species Are Necessary for Primordial Germ Cell Reprogramming into Pluripotency

<div>(a) cMyc immunofluorescence in normoxic, hypoxic, normoxia + DCA (50 <i>μ</i>M), and normoxia + resveratrol (0.5 <i>μ</i>M) cultures showing lack of reaction in SSEA1+ cell (PGC) nuclei under every condition. (b) Klf4 immunofluorescence in normoxic, hypoxic, normoxia + DCA (50 <i>μ</i>M), and normoxia + resveratrol (0.5 <i>μ</i>M) cultures showing negative reaction in SSEA1+ cell (PGC) nuclei under every condition. Positive controls are the ES cell line E14Tg2. Scale bars correspond to 25 <i>μ</i>m.</div>

Oxidative Medicine and Cellular Longevity

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Figure 6

Figure 6: Metabolic Reprogramming, Autophagy, and Reactive Oxygen Species Are Necessary for Primordial Germ Cell Reprogramming into Pluripotency 