Metabolic Reprogramming, Autophagy, and Reactive Oxygen Species Are Necessary for Primordial Germ Cell Reprogramming into Pluripotency

Figure 7

Confocal merge images of p300, H2BacK20, H3acK9, and H4acK5K8,K12,K16 immunofluorescence (in green in the nuclei) in normoxic, hypoxic, normoxia + DCA, and normoxia + resveratrol cultures, showing positive reaction in SSEA1+ cells (PGCs, membranes in red). Blue shows nuclei with DAPI stain. Colocalization is seen as light blue. p300 and histone acetylations were negative in the nuclei of normoxic SSEA1+ cells (PGCs) and positive under reprogramming conditions (DCA at 50 μM and resveratrol at 0.5 μM, all conditions maintained for 48 h). Positive controls are the human untreated Hela cell line for p300 and the NIH3T3 mouse cell line subjected to the histone deacetylase inhibitor Na⁺ butyrate at 0.25 μM for 16 hours for histone acetylations. Scale bars correspond to 100 μm in every photograph.