Protective Effects and Possible Mechanisms of Ergothioneine and Hispidin against Methylglyoxal-Induced Injuries in Rat Pheochromocytoma Cells

Figure 4

Effects of ergothioneine (EGT), hispidin (HIP), and EGT + HIP on ROS production in PC12 cells treated with 30 mM glucose and 30 μM methylglyoxal (GLU + MGO). PC12 cells were pretreated with epalrestat (EPA), aminoguanidine (AMG), ETG, HIP, and EGT + HIP for 2 h and incubated with GLU + MGO or mannitol for 48 h and 72 h. (a) Cells were collected and incubated with 5 μmol/L DCFH-DA at 37°C for 30 min, washed with PBS, and evaluated by flow cytometry. (b) Quantitative analysis indicated ROS levels in PC12 cells. Values (means ± SD of triplicate tests) without a superscript letter are significantly different ().

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