Paricalcitol Pretreatment Attenuates Renal Ischemia-Reperfusion Injury via Prostaglandin E₂ Receptor EP4 Pathway

Figure 5

Paricalcitol inhibited p65 NF-κB activation after IR injury via an EP4-dependent pathway in HK-2 cells. (a) Paricalcitol prevented the phosphorylation of p65 NF-κB in IR-exposed HK-2 cells. This inhibitory effect was reversed by AH-23848 cotreatment. (b) IR-exposed cells treated with EP4-specific siRNA and paricalcitol significantly enhanced the phosphorylation of p65 NF-κB compared with IR-exposed cells treated with siRNA control and paricalcitol. Each column represents the mean ± SEM of three independent experiments. .

(a)

(b)