Paricalcitol Pretreatment Attenuates Renal Ischemia-Reperfusion Injury via Prostaglandin E₂ Receptor EP4 Pathway

Table 1

Primer sequences for real-time PCR.

Gene	Sequence
mRANTES	Fwd: CACCACTCCCTGCTGCTTTG
	Rev: CGACTGCAAGATTGGAGCACTT
mTNF-α	Fwd: ACGGCATGGATCTCAAAGAC
	Rev: GTGGGTGAGGAGCACGTAGT
mIL-1β	Fwd: GCCCATCCTCTGTGACTCAT
	Rev: AGGCCACAGGTATTTTGTCG
mIFN-γ	Fwd: GCTACACACTGCATCTTGGCTTT
	Rev: AATGACTGTGCCGTGGCAGTAA
hCOX-2	Fwd: GATACTCAGGCAGAGATGATCTACCC
	Rev: AGACCAGGCACCAGACCAAAGA
hPGES	Fwd: CCTGGGCTTCGTCTACTCCTTT
	Rev: CCCACGAGGAAGACCAGGAA
h15-PGDH	Fwd: GAAGGCGGCATCATTATCAATATG
	Rev: GCTGAGCGTGTGAATCCAACTAT
hEP1	Fwd: TTGTCGGTATCATGGTGGTG
	Rev: ATGTACACCCAAGGGTCCAG
hEP2	Fwd: CCTGCTGCTGCTTCTCATTGTCT
	Rev: GGCTTCGGCGGTGCAT
hEP3	Fwd: AAGGCCACGGCATCTCAGT
	Rev: ACGCACATGATCCCCATAAGC
hEP4	Fwd: TCCGGCCTCAGCATCATC
	Rev: GGCTGTAGAAATAGGCATGGTTGA
mGAPDH	Fwd: TGCAGTGGCAAAGTGGAGATT
	Rev: CGTGAGTGGAGTCATACTGGAACA
hGAPDH	Fwd: GAAAAACCTGCCAAATATGATGACA
	Rev: TAGCCCAGGATGCCCTTGAG

m, mouse; h, human; Fwd, forward; Rev, reverse; RANTES, regulated on activation normal T-cell expressed and secreted; TNF-α, tumor necrosis factor-α; IL-1, interleukin-1β; IFN-γ, interferon-γ; COX, cyclooxygenase; PGES, prostaglandin E synthases; 15-PGDH, 15-hydroxyprostaglandin dehydrogenase; EP, prostaglandin receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.