Bioenergetic Changes during Differentiation of Human Embryonic Stem Cells along the Hepatic Lineage

Figure 2

(a) Immunofluorescence detection of COX4 visualized in red in the course of BG01V/hOG cell differentiation along the hepatic lineage. Nuclei are highlighted with DAPI staining shown in blue. Scale bars represent 50 μm (50 microns). (b) Quantification of cellular areas covered by mitochondrial networks in the course of BG01V/hOG cell differentiation along the hepatic lineage. (c) To ensure equal cell numbers of approximately 100,000 cells/cm² at harvest for bioenergetics analyses cells were seeded at different densities and cell numbers counted at harvest as shown in (c).

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