Bioenergetic Changes during Differentiation of Human Embryonic Stem Cells along the Hepatic Lineage

Figure 3

Measurement of acidification rate at five successive time points in the course of BG01V/hOG (hESC) differentiation along the hepatic lineage. (a) Parameters of glycolysis were determined according to rate of acidification after addition of glucose, oligomycin, and 2-DG. (b) Rate of glycolysis was measured as increase of acidification rate after addition of glucose. (c) Glycolytic capacity was measured after addition of glucose and oligomycin (, , and ; ; error bars indicate SD).

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