Bioenergetic Changes during Differentiation of Human Embryonic Stem Cells along the Hepatic Lineage

Figure 4

Measurement of oxygen consumption rate at five successive time points in the course of BG01V/hOG (hESC) differentiation along the hepatic lineage. (a) Parameters of oxidative phosphorylation were determined according to rate of oxygen consumption after addition of oligomycin, FCCP, and antimycin A. (b) ATP turnover was measured as percentagewise decrease of oxygen consumption after addition of oligomycin. (c) Reserve respiratory capacity was measured as percentage of basal respiration, after addition of FCCP. (d) Basal respiration measured in pMoles oxygen consumption per minute (, , and ; ; error bars indicate SD).

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