Blockage of NOX2/MAPK/NF-κB Pathway Protects Photoreceptors against Glucose Deprivation-Induced Cell Death

Figure 1

Inhibition of NOX2 suppresses GD-induced oxidative stress. (a)–(f) Intracellular ROS was measured with DCFH-DA identified by green fluorescence. (a)–(d) 661W cells were cultured in normal medium (a) or glucose-free medium for 8 h (b), 16 h (c), and 24 h (d). (e)-(f) 661W cells were treated with 100 μM NSC (e) or 100 μM APO (f) during the course of GD for 16 h. Scale bar = 25 μm. (g) Fluorescence intensities were measured and relative fluorescence was statistically analyzed, scale bar = 25 μm. The results are presented as means ± SEM (, compared to the control group, ^### compared to the vehicle group; one-way ANOVA and Bonferroni test).

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