Oxidative Medicine and Cellular Longevity / 2017 / Article / Fig 4

Research Article

Blockage of NOX2/MAPK/NF-κB Pathway Protects Photoreceptors against Glucose Deprivation-Induced Cell Death

Figure 4

Inhibition of NOX2 reduces cell death percentage. Cell death was evaluated by PI/Hoechst staining. PI-positive cells were identified with purple fluorescence and total cells were counter stained with Hoechst showing blue fluorescent nucleus. (a)–(d) 661W cells were cultured in normal medium or glucose-free medium for 0 h, 8 h, 16 h, and 24 h. (e), (f) 661W cells were cultured in glucose-free medium for 16 h in the presence of 100 μM NSC or APO. Scale bar = 200 μm. (g) Quantitative assessment of cell death was performed with flow cytometry. The cell death percentage was calculated as PI-positive cells/Hoechst stained cells times 100. Assays were performed in quadruplicate, and data are shown as means ± SEM ( compared to the control group, ### compared to the vehicle group; one-way ANOVA and Bonferroni test).
(a)
(b)
(c)
(d)
(e)
(f)
(g)

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at help@hindawi.com to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19. Sign up here as a reviewer to help fast-track new submissions.