Blockage of NOX2/MAPK/NF-κB Pathway Protects Photoreceptors against Glucose Deprivation-Induced Cell Death

Figure 4

Inhibition of NOX2 reduces cell death percentage. Cell death was evaluated by PI/Hoechst staining. PI-positive cells were identified with purple fluorescence and total cells were counter stained with Hoechst showing blue fluorescent nucleus. (a)–(d) 661W cells were cultured in normal medium or glucose-free medium for 0 h, 8 h, 16 h, and 24 h. (e), (f) 661W cells were cultured in glucose-free medium for 16 h in the presence of 100 μM NSC or APO. Scale bar = 200 μm. (g) Quantitative assessment of cell death was performed with flow cytometry. The cell death percentage was calculated as PI-positive cells/Hoechst stained cells times 100. Assays were performed in quadruplicate, and data are shown as means ± SEM ( compared to the control group, ^### compared to the vehicle group; one-way ANOVA and Bonferroni test).

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