BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage

Figure 6

BAG5 influences the dimerization of DJ-1 in mitochondria. Mitochondria were isolated in HEK293 cells stably expressing DJ-1-flag alone or with BAG5-Myc for examining the levels of dimer and monomer by Western blot. Tom20 acted as control protein in mitochondria. HEK293 cells coexpressing BAG5 and DJ-1 had a lower level of dimer and a higher level of monomer in mitochondria than HEK293 cells expressing DJ-1 alone (a). Quantitative data from (a) is shown (b). Results were shown as the mean ± SEM (, ).

(a)

(b)