Acute Exposure to Cigarette Smoking Followed by Myocardial Infarction Aggravates Renal Damage in an In Vivo Mouse Model

Figure 5

Effect of S and MI on nephrin and NHE-1 protein expression. Western blot analysis showing the effect of S and MI on kidney protein expression of (a) nephrin and (c) NHE-1 markers. (b, d) Histogram analysis showing the relative levels of expression of the above proteins in the 4 groups (C, S, MI, and S+MI). Results showed that S, MI, and S+MI altered protein expression for nephrin and NHE-1. Nephrin expression was significantly decreased in the S+MI group, in comparison to the S group, whereas NHE-1 protein expression increased. Quantification was done by measuring the intensity of each band by densitometry, using ImageJ software. Values represent the average fold change, normalized to GAPDH, and relative to control. Results are representatives of five independent experiments (), for each condition, reported as the mean values ± standard error of the mean (mean ± SEM). , , and indicate , , and , respectively.

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