Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study

Table 1

List of proteins significantly downregulated in elderly versus young cells (iTRAQ ratio 117/113). Statistically significant iTRAQ ratios ( value ratio and value sample ≤ 0.05) for the 40 proteins that are downregulated.

Accession/variants	ID	Description	Gene	Peptide count	Spectral count	Sequence coverage (%)	Ratio [elderly/young]	value rat	value sample	Log10 ratio
O60814	H2B1K_HUMAN	Histone H2B type 1-K	HIST1H2BK	9	131	4.76	0.170	6.27E−05	4.36E−19	−0.769
O75334-[2-6]	LIPA2_HUMAN	Liprin-alpha-2	PPFIA2	2	2	0.64	0.295	3.83E−02	5.16E−10	−0.531
P20674	COX5A_HUMAN	Cytochrome c oxidase subunit 5A, mitochondrial	COX5A	2	3	9.33	0.309	1.56E−02	2.23E−09	−0.510
P04732	MT1E_HUMAN	Metallothionein-1E	MT1E	1	9	16.39	0.310	7.22E−13	2.40E−09	−0.509
P05204	HMGN2_HUMAN	Nonhistone chromosomal protein HMG-17	HMGN2	4	11	8.89	0.330	1.89E−02	1.50E−08	−0.482
Q15075	EEA1_HUMAN	Early endosome antigen 1	EEA1	2	3	0.64	0.331	1.71E−02	1.61E−08	−0.481
Q9UKY7-[2]	CDV3_HUMAN	Protein CDV3 homolog	CDV3	3	16	11.63	0.342	1.18E−03	4.11E−08	−0.466
O75152	ZC11A_HUMAN	Zinc finger CCCH domain-containing protein 11A	ZC3H11A	1	1	1.48	0.349	2.87E−02	7.18E−08	−0.457
P17096	HMGA1_HUMAN	High mobility group protein; HMG-I/HMG-Y	HMGA1	1	19	7.48	0.352	1.56E−02	8.83E−08	−0.454
P06454-[2]	PTMA_HUMAN	Prothymosin alpha [cleaved into: prothymosin alpha, N-terminally; processed; thymosin alpha-1]	PTMA	5	24	12.61	0.357	4.01E−02	1.34E−07	−0.447
Q8NC51-[3]	PAIRB_HUMAN	Plasminogen activator inhibitor 1; RNA-binding protein	SERBP1	11	90	4.66	0.361	1.56E−02	1.82E−07	−0.442
Q13442	HAP28_HUMAN	28 kDa heat- and acid-stable phosphoprotein	PDAP1	1	1	7.18	0.363	1.23E−02	2.09E−07	−0.440
P63313	TYB10_HUMAN	Thymosin beta-10	TMSB10	2	35	13.64	0.364	1.30E−02	2.21E−07	−0.439
O00233	PSMD9_HUMAN	26S proteasome non-ATPase; regulatory subunit 9	PSMD9	1	6	5.38	0.383	2.34E−04	8.33E−07	−0.416
P05114	HMGN1_HUMAN	Nonhistone chromosomal protein HMG-14	HMGN1	3	13	8.00	0.384	1.20E−02	8.80E−07	−0.415
P62158	CALM_HUMAN	Calmodulin	CALM1	10	147	8.72	0.385	2.89E−02	9.13E−07	−0.415
P02795, P13640-[2], P80297	MT1G_HUMAN, MT1X_HUMAN, MT2_HUMAN	Metallothionein-1G, Metallothionein-1X, Metallothionein-2	MT1G, MT1X, MT2A	1	20	16.29	0.392	3.61E−03	1.47E−06	−0.406
P67936	TPM4_HUMAN	Tropomyosin alpha-4 chain	TPM4	5	48	3.23	0.401	3.64E−02	2.51E−06	−0.397
P22528	SPR1B_HUMAN	Cornifin-B	SPRR1B	4	56	8.99	0.405	2.54E−02	3.18E−06	−0.392
Q92538-[2,3]	GBF1_HUMAN	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1	GBF1	1	1	0.32	0.406	4.48E−02	3.34E−06	−0.391
P51858	HDGF_HUMAN	Hepatoma-derived growth factor	HDGF	4	12	3.75	0.429	2.37E−02	1.17E−05	−0.368
P61604	CH10_HUMAN	10 kDa heat shock protein, mitochondrial	HSPE1	12	121	7.84	0.455	2.20E−02	4.16E−05	−0.342
P07108-[2-5]	ACBP_HUMAN	Acyl-CoA-binding protein	DBI	4	50	9.20	0.460	1.86E−02	5.21E−05	−0.337
Q9C030-[2]	TRIM6_HUMAN	Tripartite motif-containing protein 6	TRIM6	2	8	1.23	0.463	1.10E−02	6.03E−05	−0.334
P20962	PTMS_HUMAN	Parathymosin	PTMS	4	11	8.82	0.468	1.44E−02	7.30E−05	−0.330
Q9GZP8	IMUP_HUMAN	Immortalization upregulated protein	IMUP	3	5	9.43	0.476	8.05E−03	1.05E−04	−0.322
Q9H299	SH3L3_HUMAN	SH3 domain-binding glutamic acid-rich-like protein 3	SH3BGRL3	3	36	10.75	0.490	1.62E−02	1.82E−04	−0.310
P62857	RS28_HUMAN	40S ribosomal protein S28	RPS28	3	20	17.39	0.491	9.21E−03	1.93E−04	−0.309
P16949-[2]	STMN1_HUMAN	Stathmin	STMN1	3	24	8.72	0.492	2.51E−02	2.00E−04	−0.308
P02765	FETUA_HUMAN	Alpha-2-HS-glycoprotein	AHSG	4	61	3.27	0.507	4.77E−02	3.38E−04	−0.295
O15212	PFD6_HUMAN	Prefoldin subunit 6	PFDN6	1	9	9.30	0.519	1.72E−02	5.20E−04	−0.285
P52926	HMGA2_HUMAN	High mobility group protein HMGI-C	HMGA2	3	8	11.93	0.528	1.02E−04	7.07E−04	−0.277
P61956	SUMO2_HUMAN	Small ubiquitin-related modifier 2	SUMO2	1	7	12.63	0.534	1.89E−02	8.65E−04	−0.272
Q9UHV9	PFD2_HUMAN	Prefoldin subunit 2	PFDN2	1	5	9.09	0.544	1.81E−02	1.16E−03	−0.265
P20929-[2,3]	NEBU_HUMAN	Nebulin	NEB	4	4	0.13	0.563	5.23E−06	2.03E−03	−0.250
P09429	HMGB1_HUMAN	High mobility group protein B1	HMGB1	5	33	5.58	0.563	9.96E−07	2.07E−03	−0.249
P62328	TYB4_HUMAN	Thymosin beta-4	TMSB4X	1	16	15.91	0.582	4.60E−03	3.41E−03	−0.235
P09497-[2]	CLCB_HUMAN	Clathrin light chain B	CLTB	7	24	3.49	0.593	1.87E−03	4.46E−03	−0.227
P35749-[2-4]	MYH11_HUMAN	Myosin-11	MYH11	1	5	0.56	0.649	4.13E−02	1.53E−02	−0.188
Q16629-[2-4]	SRSF7_HUMAN	Serine/arginine-rich splicing factor 7	SRSF7	2	13	3.78	0.651	3.09E−02	1.59E−02	−0.187