The Effects of Diabetes Induction on the Rat Heart: Differences in Oxidative Stress, Inflammatory Cells, and Fibrosis between Subendocardial and Interstitial Myocardial Areas

Figure 5

(a) Quantitative analysis of collagen volume fraction in SEN and INT areas, performed 8 weeks after diabetes induction. ^θ versus the DM SEN area; versus the CT SEN area; ^† versus the CT INT area. Data expressed in mean ± SEM. (b) Representative photomicrograph of SEN and INT areas, showing myocardial fibrosis in Masson’s trichrome stain. Blue staining indicates the myocardial fibrosis under 200x magnification. Bars: 100 μm. Western blot protein expression analysis depicting collagen I (c) and collagen III (d), performed 8 weeks after diabetes induction. versus the CT group. Data expressed in mean ± SEM in all plots.

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