Sulforaphane Prevents Angiotensin II-Induced Testicular Cell Death via Activation of NRF2

Figure 6

Nrf2 gene deletion led to a complete loss of SFN function in activating testicular antioxidant gene transcription. Nrf2 gene expression and function were determined by measuring protein levels of (a) t-NRF2 and (b) n-NRF2, along with (c) Ho1 and (d) Nqo1 mRNAs. t-NRF2, total NRF2; n-NRF2, nuclear NRF2. Data were normalised by WT Ctrl and presented as means ± SD (). versus Ctrl; versus Ang II.