Rhinacanthin C Alleviates Amyloid-β Fibrils’ Toxicity on Neurons and Attenuates Neuroinflammation Triggered by LPS, Amyloid-β, and Interferon-γ in Glial Cells

Figure 4

RC suppresses LPS-evoked microglial activation. Primary rat microglia were pretreated with 0.5 μM RC for 1 h prior to the addition of 2 μg/mL LPS for 24 h. (a) Cell culture supernatant was assessed for NO production by Griess reagent. (b–f) Quantitative PCR was used to assess transcript levels of Nos2, Il1b, Ccl2, Ccl5, and Iba1. All values are expressed as mean ± SEM (). Data were analyzed using one-way ANOVA with post hoc Dunnett’s test. ^# and ^### versus control; , , and versus LPS.

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