Rhinacanthin C Alleviates Amyloid-β Fibrils’ Toxicity on Neurons and Attenuates Neuroinflammation Triggered by LPS, Amyloid-β, and Interferon-γ in Glial Cells

Figure 7

RC diminishes CCL-2 secretion in Aβ- and IFN-γ-stimulated microglia. BV-2 cells were stimulated with Aβ (20 μM) or IFN-γ (100 pg/mL) for 24 h after 1 h incubation with RC (0.25 and 0.5 μM). (a) Cell supernatants were collected for CCL-2 measurements by ELISA. All values are expressed as mean ± SEM (). Data were analyzed using one-way ANOVA with post hoc Dunnett’s test. ^## and ns (not significant) versus control; versus Aβ. (b) Cytotoxicity was measured by LDH release assay. No significant cell death was observed.

(a)

(b)