H2S Donor NaHS Changes the Production of Endogenous H2S and NO in D-Galactose-Induced Accelerated Ageing
Verification of the in vitro accelerated ageing model and the influence of NaHS treatment on NO levels in ageing HUVECs. (a) Representative senescence-associated β-galactosidase (SA-β-gal) staining in HUVECs. (b) Statistical analysis of SA-β-gal positive cells compared with the D-gal group (). (c) Statistical analysis of cell proliferation determined by the CCK-8 assay (). (d) and (e) NO levels in the lysate and culture medium (). (f) and (g) NO levels in the lysate and culture medium with or without the CSE inhibitor PPG (). Values are the means ± SE. and .