Differential Regulation of Cardiac Function and Intracardiac Cytokines by Rapamycin in Healthy and Diabetic Rats

Figure 1

Changes in fasting serum triglyceride levels, insulin, and glucose, and serum uric acid of lean and obese rats treated with and without Rapamycin. Six-hour fasting blood collection of the indicated groups was performed for analysis of (a) serum insulin, (b) serum triglyceride levels, and (c) plasma glucose. (d) Uric acid was measured from serum samples collected at the time of sacrifice. Analysis was performed using commercially available assays (Beckman-Coulter, Brea, CA) on an automated clinical chemistry instrument (AU680, Beckman-Coulter, Brea, CA) for triglycerides, glucose, and uric acid. Insulin was measured by an ELISA kit specific for rat insulin. Values are means ± SEM. for ZL-C, ZL-Rap, and ZO-C, and for ZO-Rap. versus ZL-C, and versus ZO-C using two-way repeated measures ANOVA or Student’s t-test as appropriate.

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