Homocysteine Induces Apoptosis of Human Umbilical Vein Endothelial Cells via Mitochondrial Dysfunction and Endoplasmic Reticulum Stress

Figure 6

Inhibiting the PERK signaling pathway alleviated Hcy-induced apoptosis and the activation of NF-κb in HUVEC. (a, b, c) The expression of p-eIF2a and eIF2a, ATF4, and CHOP was determined via Western blot analysis. β-actin was selected as the loading control. (d) The expression of p-NF-κb and NF-κb was determined via Western blot analysis. (e) The apoptotic rates of cardiomyocytes exposed to different treatments were evaluated by Annexin V/PI double staining. (f) Cells were preincubated with CHOP siRNA and then stimulated with Hcy for 24 hours. The protein levels of CHOP, p-NF-κb, and NF-κb were determined via Western blot analysis. The data are presented as the means ± SD of three independent experiments. versus the control group; ^#, ^## versus the Hcy alone group or the mock + Hcy group; ^△, ^△△ versus the Tun alone group.

(a)

(b)

(c)

(d)

(e)

(f)