Small Interfering RNA Targeting Mitochondrial Calcium Uniporter Improves Cardiomyocyte Cell Viability in Hypoxia/Reoxygenation Injury by Reducing Calcium Overload

Figure 3

MCU silencing reduces mitochondrial Ca²⁺ transport. (a) MCU mRNA expression by semiquantitative qRT-PCR in silenced cardiomyocytes, versus siRNA-Neg, mean ± SEM, . (b) MCU protein expression, with β-actin used as a loading control. versus siRNA-Neg, mean ± SEM, . (c) Representative traces of mitochondrial Ca²⁺ uptake in permeabilized MCU-silenced cardiomyocytes using CG-5N Ca²⁺ indicator after 15 μM Ca²⁺ addition. The experiment was performed in the presence of 1 μM CsA. AFU, arbitrary fluorescence units. (d) Time to 50% decay analysis (T_50%) of mitochondrial Ca²⁺ transport. versus siRNA-Neg, mean ± SEM, . MCU silencing conditions were using 225 nM of siRNA-MCU at 96 h of transfection.

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