Small Interfering RNA Targeting Mitochondrial Calcium Uniporter Improves Cardiomyocyte Cell Viability in Hypoxia/Reoxygenation Injury by Reducing Calcium Overload

Figure 5

MCU silencing reduces mitochondrial oxidative stress. (a) Time-response curve of mitochondrial ROS (superoxide) production evoked by cytosolic Ca²⁺ overload with 5 μM TG in transfected cardiomyocytes. Measurements were realized over 120 min of TG treatment and ROS was detected with MitoSOX Red by flow cytometry. (b) ROS levels at 120 min of TG addition is presented as FMI fold change with respect to TG untreated cardiomyocytes. ROS production in siRNA-Neg cardiomyocytes was twofold greater than that in MCU-silenced cardiomyocytes after TG addition, , mean ± SEM, . (c) ROS production in siRNA-Neg cardiomyocytes pretreated with 5 μM Ru₃₆₀ or 1 μM CsA, measured after 120 min of 5 μM TG addition. Data are presented as described previously. and ; mean ± SEM and .

(a)

(b)

(c)