Camellia sinensis Prevents Perinatal Nicotine-Induced Neurobehavioral Alterations, Tissue Injury, and Oxidative Stress in Male and Female Mice Newborns

Figure 6

Sagittal sections in the cerebellum of (a–c) control newborns showing wide external granular layer (EGL) at day 7 which become thin at day 15 and disappeared at day 30. The molecular layer (ML) is defined at day 7, wide at day 15, and incubated by mature neurons at day 30. The Purkinje cells (PC) appear arranged in one row at day 7 and become more developed and mature at day 15 and day 30. The internal granular layer (IGL) received the migrated cells from the external granular layer, so it appears condensed at the three investigated ages. (d–f) The C. sinensis-administered group showing well-developed cerebellar fold layers similar to those of normal newborns. (g–i) The nicotine-exposed group showing a delay in the external granular layer cell migration to internal granular layer reflected by the wide external granular layer at day 7 and day 15. Purkinje cells are arranged in more than one row and appear abnormal, small, and spindle in shape. (j–l) Sections in the cerebellum of C. sinensis-supplemented nicotine-induced mice newborns showing improved histological structure. Scale bar = 50 μm.