NADPH Oxidase 2 Regulates NLRP3 Inflammasome Activation in the Brain after Traumatic Brain Injury

Figure 14

Effect of NOX2 deletion on NLRP3 inflammasome expression is conserved in both littermate and nonlittermate C57BL/6 mice at the 4-day post-TBI. Representative Western blots showing expression of NLRP3, ASC, cleaved caspase-1 (p20), and cleaved IL-1β in ctrl, WT TBI mice from Envigo (nonlittermate) and Jackson Labs (JAX, littermate to KO mice), and NOX2 KO TBI mice from JAX at the 4-day post-TBI. Densitometry quantification (normalized to β-actin and represented as fold change to ctrl) is shown to the right of the images. No differences were observed between WT mice from Envigo and JAX after TBI. Deletion of NOX2 still reduced expression of NLRP3 inflammasome components when compared to WT Jackson Labs mice at the 4-day post-TBI. (ctrl, WT Envigo, WT JAX, KO JAX, resp.). (, , ns = not significant).