NADPH Oxidase 2 Regulates NLRP3 Inflammasome Activation in the Brain after Traumatic Brain Injury

Figure 4

WT and KO mice show no differences in expression of NLRP3 inflammasome components at baseline. (a) Representative confocal images show baseline immunoreactivity of NLRP3, ASC, cleaved caspase-1 (p2), cleaved IL-1β, and NeuN in WT and KO sham mice. No differences in the expression of inflammasome components were observed between WT and KO sham mice. Scale bar represents 50 μM. (b) Quantitative RT-PCR results for ASC and IL-1β, genes that appeared slightly elevated due to sham surgery. No differences were observed between WT and KO sham mice at the 4-day postinjury in the mRNA expression of ASC and IL-1β. , 3 mice (WT, sham, and KO sham, resp.).

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