NADPH Oxidase 2 Regulates NLRP3 Inflammasome Activation in the Brain after Traumatic Brain Injury

Figure 8

NOX2 deletion leads to a decrease in NLRP3 inflammasome complex formation in the injured mouse cortex after TBI. Proximity ligation assay (PLA) demonstrates NLRP3-ASC complex formation after TBI and regulation by NOX2. Representative confocal images of Duolink in situ co-IP show red fluorescence indicative of NLRP3-ASC protein-protein interaction in the injured cortex at the 4-day post-TBI. Deletion of NOX2 attenuates NLRP3-ASC complex formation at the 4-day post-TBI in the injured cortex. Quantification of all images shows a significant increase in NLRP3 inflammasome complex formation after TBI that is significantly attenuated by NOX2 deletion (data presented as fold change relative to sham mice). mice (sham, WT, and KO, resp.). Scale bar represents 50 μM. ( sham versus WT TBI; ^## WT TBI versus KO TBI).