Dimethyl Fumarate Induces Glutathione Recycling by Upregulation of Glutathione Reductase

Figure 3

Pharmacological inhibition of glutathione reductase with BCNU is also protective when preincubated for 24 h. HT22 cells were (a) treated for 24 h with the indicated concentrations of BCNU or (b) pretreated with the indicated concentrations of BCNU for 24 h before addition of glutamate at the indicated concentrations for another 24 h. Afterwards, viability was quantified by the CTB assay (a and b). (c) Cells were treated for 24 h with 50 μM BCNU or vehicle and mRNA quantitated by real-time PCR usingβ-actin and hprt as endogenous controls. (d) HT22 cells were treated with 10 μM DMF for 24 h before simultaneous addition of BCNU and glutamate in the indicated concentrations. Viability was quantified 24 h later by the CTB assay.

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(b)

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