Immediate Early Response Gene X-1 (IEX-1) Mediates Ischemic Preconditioning-Induced Cardioprotection in Rats

Figure 1

IEX-1-mediated IPC-induced cardioprotection. (a) IEX-1 mRNA level in rat hearts post-IPC was detected by quantitative real-time PCR (). (b) IEX-1 mRNA level in rat hearts postischemia (30 min) or I/R (30 min/30 min). (c) IEX-1 mRNA level in rat hearts subjected to sham operation (Sham), I/R (30 min/3 h), or IPC prior I/R (). (d). 5 μg scrambled siRNA (csiR) or siRNA targeting IEX-1 (siIEX-1) with transfection reagent in 250 μl of saline was directly injected intramyocardially into the left ventricular muscular wall. 2 days after the injection, rats underwent I/R (30 min/24 h) or IPC prior I/R (), then hearts were excised and sliced for infarction analysis by Evans blue and triphenyltetrazolium chloride (TTC) attaining; (e) percentage of the area at risk (AAR) to the left ventricle (LV) and (f) infarct area (MI) to AAR were determined by computer-assisted planimetry. (g) Serum lactate dehydrogenase (LDH) and (h) creatine kinase (CK) activity were measured at the end of 24-hour reperfusion. , versus csiR + I/R, ^# versus csiR + IPC + I/R.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)