Immediate Early Response Gene X-1 (IEX-1) Mediates Ischemic Preconditioning-Induced Cardioprotection in Rats

Figure 5

siRNA repression of IEX-1 exacerbates myocardial infarction. 5 μg csiR or siIEX-1 with transfection reagent in 250 μl of saline (NS) was directly injected intramyocardially into the left ventricular muscular wall. 2 days after, (a) IEX-1 mRNA levels and (b) protein levels were determined. (c) 2 days after siRNA injection, rats underwent I/R (30 min/24 h). Representative images of heart infarction assessed by Evans blue and TTC staining were shown. (d) Infarct sizes are expressed as ratio of MI to AAR. (e) Serum LDH and (f) CK activity were measured at the end of 24 h reperfusion. in each group. versus sham, ^# versus NS or csiR.

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