Baicalin Ameliorates Experimental Liver Cholestasis in Mice by Modulation of Oxidative Stress, Inflammation, and NRF2 Transcription Factor

Figure 6

Baicalin enhanced nuclear translocation of NRF2 in BDL mice and increased transcription of its target genes HO-1 and GCLM. (a) Western blot images showing BDL-induced nuclear localization of NRF2 were further enhanced significantly by baicalin treatment. Western blot analyses of cytoplasmic fraction showing increased expression of NRF2 in samples with BDL pretreated with baicalin. (b) Real-time PCR analyses were performed for HO-1 and GCLM genes. BDL induced both gene expressions, and the inductions were significantly enhanced by baicalin treatment. Results are mean ± S.E.M. for each group. versus control and ^# versus BDL.

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