mTOR Inhibition Rejuvenates the Aging Gingival Fibroblasts through Alleviating Oxidative Stress

Figure 5

Rapamycin enhances the anti-inflammatory ability of human gingival fibroblasts. (a) The results of real time-PCR show the mRNA expression of inflammatory cytokines IL-6 and IL-8. Both short- and long-term treatments of rapamycin can ease the inflammatory response caused by P. gingivalis infection. (b) FACS analysis shows the reactive oxygen species (ROS) levels in hGFs. Rapamycin treatment alleviates the intracellular ROS level in hGFs caused by P. gingivalis. (c) The results of real-time PCR show (right panel) the mRNA expression of antioxidant components Cat, Sod2, and Prdx3 ( means , means , means , and means , compared with the control group).

(a)

(b)

(c)