Mitochondria-Targeted Antioxidants SkQ1 and MitoTEMPO Failed to Exert a Long-Term Beneficial Effect in Murine Polymicrobial Sepsis

Figure 8

Comparison of mitophagy and mitochondrial integrity in the liver of placebo-treated control versus SkQ1-treated groups at 24 h post-CLP. CLP mice received a total of three SkQ1/placebo injections before sacrifice at 24 h. All panels display densitometric analysis of the Western blot assay as well as representative Western blots. (a) LC3-II-to-LC3-I ratio (normalized to actin), (b) p62 (normalized to total protein), and (c) cytosolic TOM-20 release (normalized to actin). Total number of CLP mice loaded on three different gels: SkQ1 ; control (placebo) . Data as (min–max) box-and-whiskers plots. Dotted lines indicate upper/lower standard deviation calculated based on eight healthy control mice (no CLP, no treatment) that were analyzed together with the CLP mice. CM: Coumassie stained gel.

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