Ginsenoside Rg1 Ameliorates Behavioral Abnormalities and Modulates the Hippocampal Proteomic Change in Triple Transgenic Mice of Alzheimer’s Disease

Figure 6

A representative 2D-DIGE gel image of hippocampal proteins from 3xTg-AD mice with or without Rg1 treatment. Hippocampal proteins from nontreated 3xTg-AD mice and Rg1-treated 3xTg-AD mice were labeled with Cy3 or Cy5 dye, respectively ( for each group). An internal standard protein sample (a mixture of all hippocampus samples) was labeled with the Cy2 dye. The CyDye-labeled samples were combined, and the proteins were coseparated in the first dimension via IEF in 24 cm pH 3–11 nonlinear IPG strips, followed by separation in the second dimension via SDS-PAGE. Spots of interest were manually excised, digested, and subjected to identification by MALDI-TOF-MS/MS. (a) Cy2-labeled proteins as internal standards. (b) Cy3-labeled hippocampus proteins of nontreated 3xTg-AD mice. (c) Cy5-labeled hippocampus proteins of melatonin-treated 3xTg-AD mice. (d) The merged image showing Cy2-, Cy3-, and Cy5-labeled proteins. (e) Greyscale 2D-DIGE gel image showing 28 differentially expressed protein spots identified by MALDI-TOF-MS/MS (black numbers with white square) in the hippocampus of Rg1-treated 3xTg-AD mice compared with nontreated 3xTg-AD mice.

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