Measurement and Clinical Significance of Biomarkers of Oxidative Stress in Humans

Figure 1

Gating strategies in the measure of free-radical production by flow cytometry. Different leukocytes populations (lymphocytes: L, monocytes: M, and granulocytes: G) in whole blood can be identified by CD45 (b) in the live gate assigned in the forward scatter (FS) and side scatter (SS) dot plot (a) by excluding dead cells and debris. Red blood cells (RBC) can be excluded as CD45 negative (b). Platelets (Pt) can be identified by CD61 in platelet-rich plasma (PRP) (c). In activated samples, platelet microparticles (c) and leukocyte-platelet aggregates (b: Pt-G and Pt-M) are formed and Pt-G are more prone to apoptosis (G-A). After platelet activation, FS increases due to platelet aggregation inducing an increase in autofluorescence (d).

(a) Whole blood

(b) Live gate

(c) PRP

(d) PT gate