Table 3

Reversible cysteine modificationsMethodsLimitations and confoundings
S-glutathionylation GSH/GSSG SHMS, ELISA, WB
Spectrofotometric
For an accurate quantification, a specialized instrumentation is required
ROS-regulated transcription factorsMethodsLimitations and confoundings
Nrf-2, NF-kBImmunological techniques, RT-PCR‚ÄČ
ROS-generating enzymesMethodsLimitations and confoundings
NOX, MPO, XO, NOSImmunological techniques, WB, PCR, RT-PCR, enzymaticAntibody specificity
Different percentages of leukocytes’ populations
Antioxidant enzymesMethodsLimitations and confoundings
SOD, CAT, GPX, GRImmunological techniques WB, PCR, RT-PCR, enzymaticAntibody specificity
Different percentages of leukocytes’ populations

CAT: catalase; GPX: glutathione peroxidase; GR: glutathione reductase; GSH: glutathione; MPO: Myeloperoxidase; MS: mass spectroscopy; NOS: nitric oxide synthases; NOX: NADPH oxidase; PCR: reverse-transcription polymerase chain reaction; SOD: superoxide dismutase; WB: Western blot; XO: xanthine oxidase.