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Oxidative Medicine and Cellular Longevity
Volume 2017, Article ID 6792694, 15 pages
https://doi.org/10.1155/2017/6792694
Research Article

Antioxidant Treatment Reduces Formation of Structural Cores and Improves Muscle Function in RYR1Y522S/WT Mice

1Center for Research on Aging and Translational Medicine (CeSI-MeT), University G. d'Annunzio of Chieti, 66100 Chieti, Italy
2Department of Neuroscience, Imaging, and Clinical Sciences (DNICS), University G. d'Annunzio of Chieti, 66100 Chieti, Italy
3Department of General Pathology, University Estadual de Londrina, 86057-970 Londrina, PR, Brazil
4Department of Medicine and Aging Science (DMSI), University G. d'Annunzio of Chieti, 66100 Chieti, Italy

Correspondence should be addressed to Simona Boncompagni; ti.hcinu@ingapmocnob.anomis

Received 6 April 2017; Accepted 13 June 2017; Published 10 September 2017

Academic Editor: Marko D. Prokić

Copyright © 2017 Antonio Michelucci et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Table S1. This table contains the data plotted in Fig. 1 D. Data are given as mean ± SEM and are expressed as % of total number of fibers analyzed; ∗p<0.05, WT vs. RYR1Y522S/WT mice; #p<0.05, untreated RYR1Y522S/WT vs. NAC-treated RYR1Y522S/WT mice. Table S2. This table contains data plotted in Fig. 4 D-G. Data are given as mean ± SEM; ∗p<0.05, WT vs. RYR1Y522S/WT mice; #p<0.05, untreated RYR1Y522S/WT vs. NAC-treated RYR1Y522S/WT mice. In columns A and C, n = number of measurements; in columns B and D, n = number of fibers analyzed. Table S3. This table contains data plotted in Fig. S1 D-F. Data are given as mean ± SEM; ∗p<0.05, WT vs. RYR1Y522S/WT mice; #p<0.05, untreated RYR1Y522S/WT vs. NAC-treated RYR1Y522S/WT mice. Table S4. This table contains data plotted in Fig. 5 A. Data are given as mean ± SEM and are expressed as % of total number of fibers analyzed; ∗p<0.05, WT vs. RYR1Y522S/WT mice; #p<0.05, untreated RYR1Y522S/WT vs. NAC-treated RYR1Y522S/WT mice. Figure S1. Analysis of proper association between mitochondria and CRUs by EM at 4 months of age. A) Representative EM image showing the association of a mitochondrion with a -CRU. B-D) Bar plots showing the average number / area of EM section of: mitochondria (panel B); CRUs (panel C); and D) mitochondria-CRU pairs (panel C). See also Table S3. Data are given as mean ± SEM; ∗p<0.05, WT vs. RYR1Y522S/WT mice; #p<0.05, untreated RYR1Y522S/WT vs. NAC-treated RYR1Y522S/WT mice. In B-D, n = number of measurements. Scale bar: 0.1 μm. Figure S2. Analysis of relationship between relative force (% of maximum) and frequency of stimulation at 4 and 10 months of age. Force-frequency relationship curves of EDL muscles excised from 4 months old (panel A) and 10 months old (panel B) mice in which peak force at each stimulation frequency was normalized to the maximum peak force (120-Hz). Data are given as mean ± SEM. ∗p<0.05, WT vs. RYR1Y522S/WT mice; #p<0.05, untreated RYR1Y522S/WT vs. NAC-treated RYR1Y522S/WT mice.

  1. Supplementary material