Echinacoside Alleviates UVB Irradiation-Mediated Skin Damage via Inhibition of Oxidative Stress, DNA Damage, and Apoptosis
Echinacoside reduced the level of 8-OHdG in BALB/c mice and HaCaT cells exposed to UVB. (a) Paraffin-embedded sections of the mouse skin were used to immunohistological staining with 8-OHdG. The red arrow shows the epidermis thickness of BALB/c mice, and the black arrow shows 8-OHdG-positive cell. (b) Cells were pretreated with echinacoside (25, 50, and 100 μM) prior UVB exposure. After 24 h, the cells were collected to quantify 8-OHdG production using ELISA analysis. (c and d) Protein levels of 8-OHdG in cells were determined by Western blot analysis. Data are presented as means ± SD (). ## versus control group, versus UVB group.
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