Histopathological Changes in the Kidney following Congestive Heart Failure by Volume Overload in Rats

Figure 4

Confocal microscopy of cytochrome C (red fluorescence) with mitochondrial marker (green fluorescence) double immunofluorescence of the renal tubular cells in the kidney of control and ACF adult rats. Note that the cytochrome C immunostaining overlapped with the mitochondrial marker in the cytoplasm of renal tubular cells of control kidney (A, E, I and C, G, K). However, cytochrome C immunofluorescence was confined primarily within the cytoplasm after heart failure induction, where it no longer colocalized with mitochondria within renal tubular cells (B, E, J and D, H, L). Bar = 20 μm.