Estrogens Protect Calsequestrin-1 Knockout Mice from Lethal Hyperthermic Episodes by Reducing Oxidative Stress in Muscle

Figure 3

Assessment of muscle damage and blood levels of CK K⁺, and Ca²⁺ following exposure to heat stress protocol. (a–h) Histological (a–d) and immunofluorescence of EDL fibers labeled with anti-RYR1 antibody (e–h) examination of EDL muscles after exposure to the heat stress protocol in male and female CASQ1-null mice, either untreated or treated with Premarin (males) and leuprolide (females). (i) Quantitative analysis of EDL fibers presenting structural damage and contractures. See also Table S4. (j–l) Blood levels of CK in serum (j), K⁺, and Ca²⁺ in plasma (k and l) following heat stress protocol. Data are given as mean ± SEM; ; n = number of mice. Scale bars in (a–e): 10 μm (insets 5 μm).

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