Estrogens Protect Calsequestrin-1 Knockout Mice from Lethal Hyperthermic Episodes by Reducing Oxidative Stress in Muscle

Figure 6

Levels of GSH, GSSG, and 3-nitrotyrosine (3-NT) in both total hind limb and EDL muscle homogenates. (a and b) Levels of reduced (GSH) and oxidized (GSSG) glutathione (both expressed as nmol/g of tissue), measured in control conditions in hind limb muscles from male and female CASQ1-null mice, either untreated or treated with Premarin (males) and leuprolide (females). (c) GSH/GSSG ratio measured in the same specimens. (d) Representative immunoblots showing levels of 3-NT measured in control conditions in EDL muscles from male and female CASQ1-null mice, either untreated or treated with Premarin (males) and leuprolide (females). (e) Relative band densities normalized to GAPDH in the same specimens. Data are given as mean ± SEM; ; n = number of mice.

(a)

(b)

(c)

(d)

(e)