Hongjingtian Injection Attenuates Myocardial Oxidative Damage via Promoting Autophagy and Inhibiting Apoptosis

Figure 4

HJT promoted autophagic flux in H/R-induced H9c2 cardiomyocytes. (a) Western blot analysis of autophagy-related proteins: LC3-I, LC3-II, Beclin, P-ERK, ERK, P-AKT, and AKT. H9c2 cardiomyocytes were subjected to hypoxia for 24 h and reoxygenation for 2 h. (b) Protein expression levels of mTOR was determined by Western blot analysis. (c) After treatment with hypoxia for 12 h followed by 2 h reoxygenation, H9c2 cardiomyocytes were loaded with MitoTracker Green (1 μM) and LysoTracker Red (1 μM). Images were captured by confocal microscopy, scale bar, 5 μm. (d) Electron micrographs of cardiomyocytes in different groups (50000×). Black boxes indicate autophagosomes; scale bar, 0.2 μm.

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