Centella asiatica Attenuates Mitochondrial Dysfunction and Oxidative Stress in Aβ-Exposed Hippocampal Neurons

Figure 2

CAW improves mitochondrial function in Tg2576 and WT hippocampal neurons. (a) CAW treatment (50 μg/mL) increased ATP content in neurons from both genotypes (). (b) CAW attenuated bioenergetic deficits in Tg2576 neurons. Oxygen consumption rate (OCR) was significantly reduced in Tg2576 cells at baseline and after FCCP stimulation. CAW attenuated this decrease under both conditions. In WT cells, CAW treatment also significantly increased basal- and FCCP-stimulated OCR. There were no differences in OCR between genotypes or treatment groups after either oligomycin or rotenone and antimycin treatment (). (c) Tg2576 neurons exhibited diminished basal and maximal OCR relative to WT neurons, and CAW treatment attenuated these decreases. CAW also increased spare capacity in Tg2576 neurons (). , , and compared to WT control unless otherwise indicated.

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