Research Article
Heme Oxygenase-1, a Key Enzyme for the Cytoprotective Actions of Halophenols by Upregulating Nrf2 Expression via Activating Erk1/2 and PI3K/Akt in EA.hy926 Cells
Figure 2
Halophenols 1 (HP1), 2 (HP2), and 3 (HP3) protect EA.hy926 cells against H2O2-induced cell death. (a) The cytotoxicities of HP1, HP2, and HP3 toward EA.hy926 cells. Cells were, respectively, incubated with three halophenols at the indicated concentrations for 24 h and their viabilities were determined by MTT. Three compounds had no influences on cell viability at concentrations between 1.0 μM and 20 μM for 24 h. (b) H2O2 caused a concentration-dependent reduction of cell viability in EA.hy926 cells. (c) Cytoprotection of HP3 against H2O2-induced injury in EA.hy926 cells showed a dose-dependent enhancement below 10 μM. (d) Cytoprotection of 10 μM HP1, HP2, and HP3 against H2O2-induced injury in EA.hy926 cells. Pretreatment of cells with 10 μM HP1, HP2, or HP3 exhibited the significant cytoprotective actions with cytoprotective rates 63.3%, 65.8%, and 76.0%, respectively. and .
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